The Cryo-EM Workshop: A Guide to High Resolution Structure Determination was held on July 21, 2018 as part of the American Crystallographic Association Annual Meeting in Toronto, Canada. Co-organized by Lori Passmore (MRC-LMB) and Wah Chiu (Stanford U), and sponsored by Douglas Instruments, Gatan, and ThermoFisher Scientific, the one-day workshop attracted over 130 attendees.
Cryo-EM has become increasingly popular among structural biologists and is now the method of choice for structure determination of proteins larger than ~200 kDa. It has been particularly successful for proteins that are difficult to crystallize, including membrane proteins, large assemblies and multi-protein complexes. The workshop provided a detailed overview of cryo-EM specimen preparation, image processing and building/refinement of atomic models, with focus on high-resolution single particle cryo-EM, and several hands-on tutorials. Materials from the workshop can be accessed HERE and through the links below.
Note: if links below do not work, try this public box folder or this public dropbox folder for alternate access points.
Instructor | Topic |
Wah Chiu | Welcome and Introduction to Cryo-EM |
Lori Passmore | Specimen Preparation for Cryo-EM |
Chris Russo | Specimen Preparation, Data Collection and Radiation Damage || Russo&Henderson2018 |
John Rubinstein | Image Processing — Intro, Background, Implementation |
Marcus Brubaker | Image Processing — Bayesian Methods and Optimization |
Ali Punjani | CryoSparc Image Processing Tutorial |
Tom Terwilliger | Phenix tools for Cryo-EM |
Matthew Baker | Model Building in Cryo-EM || Pathwalking Tutorial |
Corey Hryc | Model Optimization Tutorial || zipfile |
Corey Hryc | Model Validation Tutorial || zipfile |
updated 07 May 2021